Effects of Dexamethasone on Secretion of Apolipoproteins A Ⅰ ,AⅡ, B100, C Ⅲ and E by Cultured HepG2 Cells
- VernacularTitle:地塞米松对HepG2细胞分泌载脂蛋白的影响
- Author:
Hao LIU
;
Zhaofeng WU
;
Bingwen LIU
- Publication Type:Journal Article
- From:
Progress in Biochemistry and Biophysics
2001;28(1):77-80
- CountryChina
- Language:Chinese
-
Abstract:
In order to obersve the effect of dexamethasone on the secretion of apolipoproteins A Ⅰ, A Ⅱ, C Ⅲ, B100 and E by cultured HepG2 cells.The apolipoprotiens contents in culture media were measured by radioimmuodiffusion assay (RID) kits developed by authors' research unit. 20-fold lyophilizely condensed culture media were used for the assays. The results showed that dexamethasone can increase the secretion of apoC and E, and inhibit the secretion of apoAⅡ , B100 and CⅢ; and the effect of dexamethasone were strengthened in a dose-dependent manner. When the concentration of dexamethasone was 5.5 × 10-5mol/L in the culture media, the secretion of apolipoprotein A Ⅰ and E increased 36.6% and 49.4%(P ＜ 0.01) respectively, while the secretion of apo AⅡ , B100 and CⅢ decreased 38.9%、 31.9% and 29.8% (P＜0.01) respectively.
