Reactive Oxygen Species are Involved in Nitric Oxide-InducedApoptosis of Neurons
- VernacularTitle:活性氧参与-氧化氮诱导的神经细胞凋亡
- Author:
Chunyang ZHANG
;
Taotao WEI
;
Hui MA
;
Yao DING
;
Dieyan CHEN
;
Jingwu HOU
;
Chang CHEN
;
Wenjuan XIN
- Publication Type:Journal Article
- From:
Progress in Biochemistry and Biophysics
2001;28(1):81-85
- CountryChina
- Language:Chinese
-
Abstract:
With redox-sensitive fluorescene probes DCFH-DA and DHR123, the formation of cytosolic and intramitochondrial reactive oxygen species (ROS) inside immature rat cerebellar granule cells during the apoptosis induced by nitric oxide donor S-nitroso-N-acetyl-pennicillamine (SNAP) was monitored by laser confocal scanning microscopy. The cytosolic and intramitochondrial ROS increase significantly after 0.5 mmol/L SNAP treatment for 1 h. Pre-treatment with the nitric oxide scavenger hemoglobin can effectively inhibit the formation of cytosolic and intrarnitochondrial ROS and protect neurons from apoptosis. Adding glutathione can also protect neurons from apoptosis, and the cytotoxity of nitric oxide increases significantly while the synthesis of glutathione is inhibited. The results indicated that ROS might be involved in NO-induced apoptosis in neural cells and glutathione might be the endogenesis antioxidant to protect neurons from oxidative injury.
