Molecular Dynamics Simulation of Docking a Novel Hirudin-likeAnti-coagulant Protein to Thrombin
- VernacularTitle:一种水蛭素类融合蛋白与凝血酶作用的动力学模拟
- Author:
Yao FAN
;
Jin WANG
;
Shan YANG
;
Xiang YANG
;
Lina ZHANG
;
Zichun HUA
;
Dexu ZHU
- Publication Type:Journal Article
- From:
Progress in Biochemistry and Biophysics
2001;28(1):86-89
- CountryChina
- Language:Chinese
-
Abstract:
Hirudin is one of the most potent anti-coagulant protein ever found, and its C-terminus is a key domain for inhibiting thrombin.In order to enhance its specificity,a novel anti-coagulant protein was constructed via fusing the C-terminus of hirudin to Annexin V, which was expected to sustain both anti coagulant activity and phorspholipid affinity. The structure of the designed protein was predicted with both molecular mechanics and dynamics. Molecular dynamics was adopted to simulate the docking interaction between the fusion protein and thrombin. The results showed the inhibitory activity of the fusion protein to thrombin.
