Overexpressed Gene YA61 Cloned from Human Gastric CarcinomaCell SGC-7901 and Its Sequencing
- VernacularTitle:人胃癌细胞一种高表达基因的克隆及序列测定
- Author:
Jinrong ZHAO
;
Xiaojun YAN
;
Fengchan HAN
;
Daxiang CUI
;
Yu HOU
;
Quanjian YAN
;
Chengzhi SU
- Publication Type:Journal Article
- From:
Progress in Biochemistry and Biophysics
2001;28(1):99-102
- CountryChina
- Language:Chinese
-
Abstract:
To clone overexpressed gene from human gastric carcinoma cell SGC-7901, DDRT-PCR technique is used with human gastric epithelial cell GES-1 as control. After cloned into pGEM -T vector, YA61, one of the overexpressed genes, was analyzed by dot blot and was sequenced then. The sequence gotten was then compared to GenBank data and analyzed by NCBI ORF Finder. Dot blot results showed that the gene YA61 was overexpressed in human gastric carcinoma cell SGC-7901. NCBI's sequence similarity search indicated that the gene YA61 was a new gene sequence. Open reading frame analysis demonstrated that the gene YA61 had one complete open reading frame. In conclusion, the gene YA61 was a new gene sequence that was overexpressed in human gastric carcinoma cell SGC-7901.
