Relationship Between Expression of Apoptosis-modulating Proteins and Multidrug Resistance in K562/VCR Cells
- VernacularTitle:凋亡调节蛋白的表达与K562/VCR多药耐药性的关系
- Author:
Yusheng ZHU
;
Yuan LU
;
Guowei LIN
;
Rongxing ZHANG
;
Zhenghong YANG
;
Yuangang LU
- Publication Type:Journal Article
- From:
Fudan University Journal of Medical Sciences
2001;28(1):32-34
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To explore the Relationship between expression of apoptosis-modulating proteins amdmultidrug resistance in K562/VCR cells. Methods Irnmunocytochemical methol and western blot wereused to analyze the expression of apoptosis-modulating proteins (Bcl - 2, Bcl-XL, Bax, Bak ) in multidrugresistant cell line K562/VCR and drugsensitive cell line K562. Results The positive cell rates ofapoptosis-suppressing protein Bcl-2 and Bcl-XL in K562/VCR were (40.0 ± 8.0) % and (60.0 ± 10.0) % .While the rates in K562 were (1.0 ± 0.3) % and (20.0 ± 4.0) %. There was significant difference in thepositive cell rates of Bcl - 2 and Bcl - XL between K562/VCR and K562 ( n = 3, P ＜ 0.05 ). It was alsofound there was no significant difference in expression of Bax between K562/VCR and K562. Furthemore,Bak was not expressed in both K562/VCR and K562 or the expression was very low. Conclusions Wesuggest that Bcl-2 and Bcl-XL play important roles in multidrug resistance in K562/VCR, while Bax and Bakmight not be important.
