Manufacture of Complementary DNA Arrays on Amino-modified Slides
- VernacularTitle:用氨基修饰的载玻片制作cDNA微阵列
- Author:
Bin ZHU
;
Fenxiang JIN
;
Jianlong ZHAO
;
Yue SUN
;
Jifeng CHEN
;
Xintai ZHAO
;
Yuansen XU
- Publication Type:Journal Article
- From:
Progress in Biochemistry and Biophysics
2001;28(1):121-124
- CountryChina
- Language:Chinese
-
Abstract:
The use of rnicroarrays of oligonucleotides or cDNA is considered to be a promising approach for DNA and RNA sequence analysis, diagnostics of genetic diseases, gene polymorphism studies and analysis of gene expression. To manufacture cDNA microarrays the samples were printed onto glass microscope slides treated with poly-L-lysine, and then the slides were processed by heat and UV light treatment to attach the cDNA sequence to the glass surface. But the immobilization efficiency of cDNA on the glass surface was low. A simple procedure for manufacture cDNA microarrays on a slide treated with 3-aminopropyltrimethoxysilane is described. The efficiency for attaching cDNA to the amino-modified slides is greater than that to the slides treated with poly-L-lysine. The cDNA microarray made by the amino-modified slides is stable for use in 80℃, 75 % humidity, 3 600Lx light, exposure in air, respectively.
