Improvement of Glutamic Decarboxylase Radioassay and Its Apply
- VernacularTitle:谷氨酸脱羧酶放射测量法的改良及应用
- Author:
Yuanyuan HU
;
Shanshu HE
- Publication Type:Journal Article
- From:
Progress in Biochemistry and Biophysics
2001;28(1):118-120
- CountryChina
- Language:Chinese
-
Abstract:
The radioassay of glutamic decarboxylase (GAD) was modified by taking NaOH as trapped agent instead of phenylethylamine. The results showed that the coefficient of variation (CV) within same sample was 9.6 % and the radioactivity remains stable after 72 hours if use NaOH as trap agent. It is significantly stable than use phenylethylamine as trap agent, which the CV was 31.9 % and the radioactivity decreased 47% within the first hour and decreased to background after 6 hours. The reabsorption experiment shows over 80 % of 14CO2 can be reabsorption by NaOH within 6 hours. It is suggested that NaOH is a much better trap agent than phenylethylamine and the sensitivity can increase 1.66 folds. Using this method the GAD activity in 0.39～ 17.8 rng of brain tissue can be measured and it is success in determine the GAD activity both in rat brain tissue and cultured neurons.
