A Comparison Study of Luminescent Characters of Two Recombinant Mycobacteriophages
- VernacularTitle:重组分枝杆菌噬菌体检测分枝杆菌的发光研究
- Author:
Bin Lü
;
Shunqing XU
;
Zhijun FU
;
Zhifei CHEN
;
Kaichang LI
;
Yikai ZHOU
- Publication Type:Journal Article
- From:
Progress in Biochemistry and Biophysics
2001;28(1):108-112
- CountryChina
- Language:Chinese
-
Abstract:
To compare the luminescent characters in different bacteria of two different recombinant mycobaceriaphages by using biolimenescent methods in order to understand the differences between sensitivity and specificity of these phages, and to set up methods to use recombinant mycobacteriaphages in detecting drug suscepbility of mycobacteria. Result showed that both two phages have high light production in action with mycobacterium selectively and have almost no light production with E. coli , the difference is very obvious. Among different mycobacterium, BCG has the highest light production and mycobacterium tuberculosis has the lowest light production. The sensitivity of Phage 88 is higher than Phage 40, the difference is obviously. It can be considered that both recombinant mycobacteriaphages can detect mycobacterium specifically, but Phage 88 is more suitable for clinical usage.
