Detection of IgG2b Monoclonal Antibodies Against LDH-C4 in Sera of Mice Bearing Hybridoma Backpack Tumors by Quantitative ELISA
- VernacularTitle:定量ELISA检测小鼠血清中抗LDH-C4 IgG2b抗体的水平
- Author:
Ling SUN
;
Shengmin HUAG
;
Xiaolei WANG
;
Yongzhen ZHANG
;
Xiaomei CAO
;
Kunlong BEN
;
Jinju XU
- Publication Type:Journal Article
- From:
Journal of Kunming Medical University
2001;22(1):30-33
- CountryChina
- Language:Chinese
-
Abstract:
The levels of IgG2b monoclonal antibodies against LD H-C4 in sera of mice bearing hybridoma backpack tumors secreting anti-LDH-C4-IgG2b were detected by quantitative ELISA． The accuracy between batches is 7.04%～l3.30 %, the intra-assay variation is 3.6l%～l0.20%． Standard curveof monoclonal lgG2b was well correlated (r=0.962 884～0.996 795)． The sensitivit y of the assay reach e dup to0.0l?mg/L． The present modified ELISA offers a reproducible, se nsitive, specific method in determination of antigen-specific IgG2b antibody in sera．
