Detection of human papilloma virus type 16 and 18 E6 gene in exfoliative cervical cell
- VernacularTitle:宫颈脱落细胞标本中人乳头瘤病毒16、18型E6基因的检测
- Author:
Zhenrong LI
;
Jie ZHANG
;
Xiaoying LI
- Publication Type:Journal Article
- From:
Basic & Clinical Medicine
2001;21(1):55-57
- CountryChina
- Language:Chinese
-
Abstract:
To observe possibility whether exfoliative cervical cell takes the place of cervical tissues for detection of human papilloma virus 16 and 18 types (HPV16 and HPV18). Polymerase chain reaction-restrictive fragment length polymorphism was used for analysis cervical tissues, single and double oxfoliative cervical cell samples of 127 cases of woman patients. Positive rates of HPV16 E6 Gene in single and double oxfoliative cervical cell are 34.64% and 40.73%, for HPV18 E6 gene 17.32% and 22.04%. In cervical tissues, 41.73% of HPV16 and 22.83% HPV18 special E6 genes was detected. There is no difference between cervical tissues and double oxfoliative cell samples. It is proved that double oxfoliative cervical cell sample can be used for detection of infection of HPV 16 and HPV 18 instead of cervical tissues.
