Effect on D1-Like and D2-Like agonist on GABAA-receptor in rat dorsal root ganglion
- VernacularTitle:多巴胺受体两个亚家族对大鼠背根神经节GABAA受体的影响
- Author:
Qin LI
;
Hanlang CHEN
;
Zhiwang LI
- Publication Type:Journal Article
- From:
Basic & Clinical Medicine
2001;21(1):36-39
- CountryChina
- Language:Chinese
-
Abstract:
The experiment was performed on neurons freshly isolated from rat DRG by using whole cell patch clamp techniques. When 60 cells responded to GABA were treated with SKF38393, a selective agonist of dopamine Dl receptor, it was found that except one cell which exhibited a slight potentiation( l0.0%,l/60), the inhibition by SKF38393 of GABA-activated current was predominant (88.33%,53/60), while the remainders were of no effect( l.66%,6/60). In 50 neurons tested , when the neurons were treated with R(-)-NPA prior to the application of GABA for 30sec, It was found that GABA-activated membrane currents were inhibited (76%,38/50) , enhanced (2%,l/50) , no effected markedly (22%,11/50). The effect of SKF38393 and R(-)-NPA was dependent on the concentration of SKF38393 and R(-)-NPA . By intracellular application of 10-4mol/L H7, a potent inhibitor of protein kinase , via micropipette the inhibition of SKF38393 and R(-)-NPA was abolished apparently as compared with the control .
