THE DETECTION OF THE EVIDENCE OF ENGRAFTMENT AFTER NONMYELOABLATIVE ALLOGENEIC PERIPHERAL BLOOD STEM CELL TRANSPLANTATION

VernacularTitle:非清髓异基因外周血造血干细胞移植后供体细胞植入证据的研究
Author: Changlin YU ; Huisheng AI ; Bo YAO
Publication Type:Journal Article
From: Medical Journal of Chinese People's Liberation Army 2001;26(1):16-17
CountryChina
Language:Chinese
Abstract: This paper investigate the methods to detect engraftment rate of the four patients with hematological disorders who accepted nonmyeloablative allogeneic peripheral blood stem cell transplantation(NAPBSCT). To find out the best method, their engraftment rates were detected serially at different time after NAPBSCT by means of either FISH, or conventional chromosome analysis combined with R-banding analysis concurrently.The results were carefully compared with one another. All these four sex-mismatched cases were engrafted partially,and two of them changed to full engrafment. The results show no statistically significant difference in 3 groups (conventional method, FISH for hypermetaphase, FISH for interphase nuclei). But the results strongly indicate that FISH is a rapid, precise, objective,and reliable menthod for detection of the engraftment rate,and it is suitable for sex-mismatched NAPBSCT.