Collagen Distribution and its Quantitative Variation in the Aortic Adventitia of Spontaneously Hypertensive Rats
- VernacularTitle:自发性高血压大鼠胸主动脉外膜胶原分布及含量的变化
- Author:
Ting TAO
;
Dingliang ZHU
;
Langsheng GONG
- Publication Type:Journal Article
- From:
Chinese Journal of Hypertension
2001;9(1):50-52
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study collagen distribution and its quantitative variation in t he aortic adventitia of spontaneously hypertensive rats Methods: Sirus red stain was used to observe collagen distribution in aortic adventitia；Chloramine-T oxidation assay was employed to quantitate the collagen of aortic adventitia；Immuno-histochemical staining was adopted to detect type Ⅰ、Ⅲ collagen synthetic characteristic of vascular adventitial fibroblasts. Results: Collagen of rat aoritc wall mainly distributes in adventitia；SHR shows more collagen deposition in adventitia than WKY at the age of 8weeks and 24 weeks；The immuno-histochemical staining of type Ⅰ、Ⅲ collagen in vascular a dventitial fibroblasts is positive. Conclusions: All those results indicate that vascular adventitia ma y be involved in hypertensive vascular remodeling.and vascular adventitial fibroblasts can synthe size collagen type Ⅰ and Ⅲ.
