Effects of Benazepril and Losartan on Proliferation and Collagen Synthe sis of Cultured Cardiac Fibroblasts from SHR
- VernacularTitle:Benazepril和Losartan对培养SHR心肌成纤维细胞增殖及合成胶原影响
- Author:
Liangdi XIE
;
Shuilong CHEN
;
Changsheng XU
;
Huajun WANG
;
Kegui WU
- Publication Type:Journal Article
- From:
Chinese Journal of Hypertension
2001;9(1):62-64
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of angiotensinⅡ(AngⅡ), Benazepril(Bena) and Losartan on proliferation and collagen synthesis of cultured rat cardiac fibroblasts(CFb) derived from SHR (CFbSHR) and WKY (CFbWKY). Methods CFb derived from 12-week-old SHR and WKY was cultured by outgrowth of tissue block. Cell proliferation of CFb was determined by direct cell counting .3H-Proline incorporation of CFb was measured after incubation with AngⅡ,Bena and Losartan. Results In serum free(0.4%FCS) medium, cell number of CFb derived from SHR and WKY were not influenced by AngⅡ(10-10mol～10-6mol)and Bena(10-9mol～10-5mol). Increased 3H-proline incorporation wa s induced by AngⅡ in a concentration dependent manner. Benazepril caused an decrease in 3H-Proline incorporation in CFb derived from SHR and WKY. The increased collagen synthesis induced by AngⅡ(10-7mol) was inhibited by Losartan((10-10mol～10-6mol） i n a concentration dependent manner. Conclusion Proliferation of CFb were not influenced by AngⅡ and Bena. Collagen synthesis of CFb was promoted by AngⅡ and inhibited by Bena. Collagen synthesis of CFb in duced by AngⅡ was inhibited by losartan.
