Effect of human growth hormone on cell cycle kinetics in colonic cancer cells
- VernacularTitle:人生长激素对人结肠癌细胞LS-174-T增殖周期的影响
- Author:
Xiaoping QI
;
Jieshou LI
;
Che CHEN
- Publication Type:Journal Article
- From:
Parenteral & Enteral Nutrition
2001;8(1):8-10
- CountryChina
- Language:Chinese
-
Abstract:
Objectives:The effects of human growth hormone (hGH) on cell cycle kinetics were studied in human colonic cancer cell line, LS-174-T cell. Methods: The cells were cultured in RPMI－1640 medium for 24 hours with hGH at concerntration of 50, 100, 200 ng/ml with or without cisplatin. Cell cycle kinetics was analyzed by flow cytometry. Results: The significant effects of hGH on cell cycle kinetics were found in the cell line. Percentage of S phase increase (P<0.01) and percentage of G2/M phase decreased (P<0.01).DNA Index (DI) didn't increase.The cells had a lot of apoptosis and percentage of S phase decreased (P<0.01) with addition of cisplatin. Conclusions: In vitro hGH may induce the proliferation of colonic cancer cells. Cisplatin may suppress the stimulatory effects of hGH on S phase and increase the apoptosis of colonic cancer cells.
