Identification of melatonin receptor in human embryonic peripheral tissue
- VernacularTitle:人胚胎外周组织褪黑素受体的鉴定
- Author:
Zhi-Min LIU
;
Jun-Jie ZOU
;
Yu-mei SHEN
;
Zu-Qian LU
- Publication Type:Journal Article
- From:
Academic Journal of Second Military Medical University
2001;22(1):8-11
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To determine protein binding characteri stic and signal transmission pathway of melatonin（Mel） receptor(MR) in human e mbryonic peripheral organ tissues. Methods: MR was measured by radio ligand-binding assay and the effect of GTPγS on melatonin specific bindi ng was studied. Results: Mel specific binding sites were det ermined in 16 kinds of human embryonic tissue and this binding could be inhibit ed by GTPγS, supporting the theory that MR is coupled to inhibitory G-proteins system. Conclusion: MR is measured in human embryo tissue, the se results provide experimental data for elucidating the mechanism of the effect of Mel.
