Detecting melatonin receptor in thyroid of human embryo with immunohistochemistr y and in situ hybridization
- VernacularTitle:用原位杂交及免疫组化方法显示人胚胎甲状腺褪黑素受体
- Author:
Zhongan SUN
;
Zuqian LU
;
Zhimin LIU
- Publication Type:Journal Article
- From:
Academic Journal of Second Military Medical University
2001;22(1):15-17
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To make it clear whether there exists m elatonin receptor in the thyroid of human embryo. Methods: Thyr oid was collected and sliced up to be stained with methods of immunohistochemis try and in situ hybridization. Results: The thyroid tissue w as p ositively dyed, melatonin receptor mt1 and MT2 were with both immunohistoche mistry and in situ hybridization while brown granules dep osited in the membrane, plasma and nuclear of the thyroid cell were with the imm unohitochemistry. Conclusion: There exists melatonin rece ptors in human embryo thyroid, either mt1 or MT2, and they exist in the memb rane, plasma and nuclear.
