The Effect of PPAR gamma2 Pro12Ala Polymorphism on Diabetic Nephropathy in Type 2 Diabetes.
- Author:
Dong Min KIM
1
;
Hyun Lee KIM
;
Bum Yun KIM
;
Byung Chul SHIN
;
Jong Hoon CHUNG
;
Hak Yeon BAE
;
Byung Rae LEE
Author Information
1. Department of Internal Medicine, Chosun University of Medical School, Gwangju, Korea. hyunkim@chosun.ac.kr
- Publication Type:Original Article
- Keywords:
PPAR gamma2;
Polymorphism;
Diabetic nephropathy
- MeSH:
Blood Pressure;
Cholesterol, LDL;
Codon;
Creatinine;
Diabetic Nephropathies*;
Genotype;
Humans;
Mass Screening;
Peroxisome Proliferator-Activated Receptors*;
Proteinuria;
Triglycerides
- From:Korean Journal of Nephrology
2004;23(5):729-737
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Recently, the PPAR gamma2 Pro12Ala polymorphism has been associated with type 2 diabetes and diabetic nephropathy. In the present study, we investigated the association between Pro12Ala polymorphism and diabetic nephropathy in korean subjects. METHODS: A total of 180 patients with type 2 diabetes and 100 normal controls were enrolled in this studies. Screening for mutation at codon of PPAR gamma2 were carried out by PCR-RELP analysis. Also, we measured important covariables, such as duration of diabetes, blood pressure, renal function and serum lipids. RESULTS: In PCR-RELP, it showed that there were no difference in the PPAR gamma2 genotype frequencies between diabetic subjects (Pro/Pro: 80.5%, Pro/Ala: 19.5%) and normal controls (88%, 12%). However, it showed that there were significant difference in the PPAR gamma2 genotype frequencies between diabetic subjects with nephropathy (Pro/Pro: 74.5%, Pro/Ala: 25.5%) and diabetic subjects without nephropathy (87.8%, 12.2%) (p=0.040). In diabetic subjects, Pro/Ala genotypes were significantly different from Pro/Pro regarding serum creatinine, 24 hour proteinuria, systolic pressure, and LDL-cholesterol. In diabetic nephropathy, genotypes with Pro/Ala significantly increased serum creatinine (2.7+/-0.41 vs 1.7+/-0.68 mg/dL), 24hour urine protein (median+-SE: 1.9+/-1.02 vs 0.9+/-0.44 g/day), systolic pressure (161+/-27.8 vs 152+/-32.4 mmHg), LDL cholesterol (134+/-30.4 vs 125+/-20.0 mg/dL), and triglyceride (151+/-86.5 vs 135+/-60.9 mg/dL) than genotypes with Pro/Pro. CONCLUSION: Our results suggested that Pro12Ala Polymorphism of the PPAR gamma2 gene may be associated with diabetes with nephropathy in korean patients.
