The number and function analysis of T cell from mice immunized with MIF gene-modified tumor vaccine
- VernacularTitle:MIF基因修饰的瘤苗免疫小鼠后T细胞数量和功能的分析
- Author:
Yanjun LIU
;
Yeping TIAN
;
Xuetao CAO
;
Minghui ZHANG
;
Yongjian LI
;
Zhengfang ZHOU
;
Lingli ZHENG
- Publication Type:Journal Article
- From:
Academic Journal of Second Military Medical University
2001;22(1):32-35
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the role of T cell in the antitumor immune responses induced by MIF gene-modified tumor vaccine. Methods: MIF gene was transferred into FBL3 erythroleukemia cel l by adenovirus carrier and a new type of tumor vaccine was prepared. The chang es of the number and the function of T cell in spleen and lymph node was observe d. Results: After the mice were immunized with MIF gene-m odified FBL3 vaccine, the number of lymphocyte in spleens and lymph nodes increa sed markedly and the specific CTL activities of splenocytes also increased great ly. FACS analysis showed that the CD3+, CD4+, CD8+ T cells and CD28 posi tive cells in draining lymph nodes of MIF-FBL3 group mice increased more marked ly than that of control groups. When the wild type FBL3 cells were injected into the mice immunized with MIF gene-modified FBL3 vaccine, the growth of tumors w ere obviously inhibited and the survival rate of the mice was increased. Conclusion: It is suggested that MIF gene-modified tumor vaccine can induce specific antitumor immune responses mediated by T cells and may be a candidate for gene therapy of tumor.
