Alteration of Bcl-2, Bcl-x and Bax protein expression following fluid per cussion brain injury in rats
- VernacularTitle:大鼠液压脑损伤后Bcl-2、Bcl-x和Bax蛋白表达的改变
- Author:
Chun LUO
;
Cheng ZHU
;
Yicheng LU
;
Jiyao JIANG
- Publication Type:Journal Article
- From:
Academic Journal of Second Military Medical University
2001;22(1):54-56
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the alteration of bcl- 2 gene family in the rat brain and the molecular mechanism of neuronal apoptosis following traumatic brain injury. Methods: Male Sprague -Dawley rats were subjected to lateral fluid percussion brain injury(FPI) of mo derate severity. Bcl-2, Bcl-x and Bax protein expression was detected by immun ohistochemistry. Results: (1) The immunoreactivity of Bcl-2 and Bcl-x protein decreased in the hippocampus ipsilateral impact site as early as 6 h post-injury, and this was the main cause of down-regulation of the ratio of Bcl-2+Bcl-x to Bax. (2) During 1-3 d after injury, the Bax protein express i on increased significantly, while the Bcl-2 and Bcl-x protein expression decre ased relatively slow. The decreased ratio of Bcl-2+Bcl-x to Bax was mainly due to the Bax up-regulation. Conclusion: The bcl-2 gene family is involved in neuronal apoptosis after FBI, and the protein expression alteration of the family members leads the neuronal cell to apoptosis.
