Examination of Rb、P53、C-myc and HPV genes in tissue of laryngeal squamous cell carcinoma and their significance
- VernacularTitle:喉癌组织Rb、P53、C-myc和HPV基因测定及意义
- Author:
Fengan LIU
;
Xiaofeng GUO
- Publication Type:Journal Article
- From:
Chinese Archives of Otolaryngology-Head and Neck Surgery
2001;8(1):52-55
- CountryChina
- Language:Chinese
-
Abstract:
The genes of P53,retinoblastoma (Rb),C-myc and human papilloma virus (HPV) were determined by PCR method in specimens of 33 patients with laryngeal carcinoma and 4 normal laryngeal tissues as a control group.There were 23 males and 10 females,ranging in age from 40 to 11 years old.Results showed that the mutation rate of P53 and Rb gene were 69.69%(23/33) and 48.48%(16/33),respectively;c\|myc gene amplification rate and HPV insertion rate were 58.62% (17/29) and 72.72%(24/33),respectively.There were significant difference(P＜0.05)in comparison between positive and negative expression above these genes.The expression rate were 50.00%(12/24),45.83%(11/24)and 66.67%(16/24)of C-myc,Rb and P53 genes respectively in 24 cases who were HPV positive of laryngeal carcinoma.The positive rates were 73.68%(14/19),63.16%(12/19),57.89%(11/19)and 84.21%(16/19) of HPV,C-myc,Rb and P53,respectively in 19 cases who have heavy smoking (20/day,20 years smoking history).Above results suggested that there were closed relationship in the C-myc gene activation and P53,Rb genes inactivation with smoking and HPV infection.It was believed that carcinogen factors on laryngeal carcinoma was the result of factors,genes and stages interaction.
