Effects of Cobalt in different concentration on activity of myocardium enzymes in medium of cultured myocardium cell
- VernacularTitle:不同剂量钴对大鼠心肌细胞培养液中心肌酶活性的影响
- Author:
Yi SHI
;
Zhongying LIU
;
Yaqin TAN
- Publication Type:Journal Article
- From:
Journal of Jilin University(Medicine Edition)
2001;27(1):31-32
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To find the threshold value concentration of cobalt causing myocardium cell damage.Methods:Cobalt in different concentrations (10 μg/ml,20 μg/ml,40 μg/ml,80 μg/ml) were added into the medium of cultured myocardium cell of rat (1～3 days old) in vitro.Then the activities of GOT,CK,LDH,HBDH in the medium were measured.Results:The results showed that when cobalt concentration was 10～20 μg/ml,the activity of GOT,CK,LDH,HBDH did not change markedly.But when cobalt concentration was more than 40 μg/ml,the activities of those enzymes increased significantly.Conclusion:The studies demonstrated that the activities of enzymes in the medium of cultured myocardium cell could increase with the difference of coblat concentrations.The threshold value concentration of cobalt which could cause myocardium cell damage is 40 μg/ml.
