Ultrastructure changes of osteosarcoma cells inactivated by alcohol in different concentrations
- VernacularTitle:不同浓度酒精灭活后的国人成骨肉瘤细胞超微结构的变化
- Author:
Feng NIU
;
Guishan GU
;
Yi ZHAO
;
Daming QIN
;
Xinxiang XU
- Publication Type:Journal Article
- From:
Journal of Jilin University(Medicine Edition)
2001;27(1):49-51
- CountryChina
- Language:Chinese
-
Abstract:
Objective：To study the ultrastructure changes of osteosarcoma cells(OS-732) inactivated by alcohol of two different concentrations and clinical application value.Methods：Osteosarcoma cells(OS-732) were inactivated by 75% and 95% alcohol and observed by light and electron microscope.And its viability was detected by MTT method. Results：Cell internal structure changed significantly and irreversible damage formed. MTT method proved that inactivated cells had no viability.Conclusion：These two inactivation methods were effective. Cell internal enviroment was damaged very seriously and cell was led to death. These two inactivation methods could provide choices for clinical limb protective operation.
