Stimulating proliferation of aorta smooth muscle cells of rat by rat urotensin Ⅱ
- VernacularTitle:尾加压素Ⅱ对血管平滑肌细胞增殖的影响
- Author:
Yonggang ZHANG
;
Yongfen QI
;
Chunfang XIA
;
Yongzheng PANG
;
Jun YANG
;
Zhaokang ZHANG
;
Chaoshu TANG
- Publication Type:Journal Article
- From:
Chinese Pharmacological Bulletin
2001;17(2):155-157
- CountryChina
- Language:Chinese
-
Abstract:
AIM To investigate effect of urotensin Ⅱ (UⅡ)on proliferation of aort a smooth muscle cells (ASMC)of rat and study the signal transduction pathway o f it. METHODS In cultured ASMC of rat, UⅡ was used to stimulate proliferation of these cells and levels of [3H]-TdR incorporation were used to evaluate the speed of DNA synthesis, and different inhibitors were used to s tudy the action of different signal transduction pathway of mitogenic effect of UⅡ on VSMC. RESULTS 1×10-9~1×10-7 mol*L- 1 UⅡ caused marked concentration-dependent increasing of [3H]-TdR i ncor poration of ASMC. [3H]-TdR incorporation of 1×10-9,1×10-8 and 1×10-7 mol*L-1 UⅡ were 22%(P<0.05), 57%(P<0.01)and 65%(P<0.01)higher than control. Nicardipine, H7, W7 and PD98059 , which are inhibitors of calcium channel, PKC, CaM-PK and MAPK respectively, inhibited the effects of UⅡ obviously, with the inhibitory rate by 55%(P< 0.01), 27%(P<0.01),18%(P<0.05)and 16%(P<0.05)respectively . CONCLUSION UⅡ is a strong mitogen for VSMC and the mitogenic e ffect of UⅡ is probably mediated by Ca2+, PKC, CaM-PK and MAPK signal tr ansduction pathway.