Effect of desipramine on proliferation inhibition and apoptosis induction in rat glioma C6 cells
- VernacularTitle:地昔帕明对脑胶质瘤C6细胞的增殖抑制和凋亡诱导作用
- Author:
Hong QI
;
Hongzhuan CHEN
;
Jumei FENG
;
Yuyan SUN
;
Zhengjun JIN
- Publication Type:Journal Article
- From:
Chinese Pharmacological Bulletin
2001;17(2):161-164
- CountryChina
- Language:Chinese
-
Abstract:
AIM To study the effect of desipramin e (DMI) on proliferation inhibition and apoptosis induction of rat glioma C6 cel ls. METHODS Cell proliferation w as measured by MTT colorimetric assay and cells undergoing apoptosis were determ ined by electron microscope and flow cytometry. The expression of bcl-2 was eva luated by immunohistochemistry. RESULTS DMI could result in the c oncentration-dependent inhibition of C6 cell proliferation and lead to arrest i n G0~G1 phase of cell cycle. The value of IC50 and 95% confidence lim its were 20.7(17.3~24.2) μmol*L-1.DMI(40 μmol*L-1)-indu ced apoptosis showed classical apoptotic morphology and the hypodiploid peak ap peared on the histogram of FCM in a concentration-dependent manner, which could be abrogated by cycloheximide(1.8 μmol*L-1). Meanwhile, DMI (10 μmol *L-1) could down-regulate the expression of apoptosis associated gene b cl-2. CONCLUSION DMI could inhibit cell proliferation in a con centration dependent manner and induce typical apoptosis of C6 cells.