Effect of quercetin on cell cycle and nitroxide in hydrogenperoxide-induced of endothelial cells
- VernacularTitle:槲皮素对过氧化氢诱导的血管内皮细胞周期及NO水平的影响
- Author:
Rong LIN
;
Juntian LIU
;
Xu LI
;
Wei CHEN
;
Yucong YANG
;
Xiaoli CHENG
- Publication Type:Journal Article
- From:
Chinese Pharmacological Bulletin
2001;17(2):211-213
- CountryChina
- Language:Chinese
-
Abstract:
AIM To study the effects of quercetin (Que) on cell cycle and nitric oxide in H2O2-induced the human umbilical vein endothelial cell line(ECV-304). METHODS The experiment were performed in culture of H2O2-induced human umbilical vein endothelial cell line(ECV-304) in vitro. Cell viability was assessed by MTT assay and cell cycle was observed by flow cytometry. Nitroxide(NO) of ECV-304 was monitored as NO2- with colorimetry. RESULTS H2O2 inhabited the ECV-304 prolifteration. Preincubation of ECV-304 with Que for 24 h before H2O2 exposure significantly increased the cell viability and S-phase and G2M-phase cells. Que reduced lactate dehydrogenase(LDH) and increased the level of nitric oxide in H2O2-induced ECV-304. CONCLUSION These results demonstrate that Que can produce the protective action on H2O2-induced cultured ECV-304 and its effect of action may be related to level of nitric oxide.
