Fluorescent measurements of H+/K+ exchange in primary cultured inner medullary collecting duct cell monolayers of rabbit kidney
- VernacularTitle:原代培养家兔肾脏内髓集合管细胞单层H+/K+交换测定
- Author:
Qianming XIA
;
Jian HUANG
;
Zhenliang XIAO
;
Guisheng QIAN
- Publication Type:Journal Article
- From:
Chinese Pharmacological Bulletin
2001;17(2):230-232
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish the fluorescent measurement technique for H+/K+ exchange in primary cultured IMCD cell monolayers of rabbit kidney. METHODS To measure H+/K+ exchange in the primary cultured IMCD cell monolayers under different CO2 concentrations by the BCECF/AM fluorescent probe.RESULTS ［pH］i recovery rate after acid loaded in the respiratory acidosis group was 0.0620±0.012，and was significantly higher than that of the control group（0.0504±0.009，P<0.05）;which in the respiratory alkalosis group was 0.0476±0.007, there was no signifacant difference between ALG and CG （P>0.05）. CONCLUSION The fluorescent measurements of H+/K+ exchange in primary cultured IMCD cell monolayers of rabbit kidney has many benefits which are convenient manipulation, stable and credit result, economy and safety , this method should further be popularized.
