Effects of Ganoderma Polysacchrides on PKC and PKA Activities in Murine T Cells
- VernacularTitle:灵芝多糖对小鼠T细胞蛋白激酶A和蛋激酶C活性的影响
- Author:
Mingchun LI
;
Dongsheng LIANG
;
Ziming XU
;
Linsheng LEI
;
Qingbiao WANG
;
Shuqin YANG
;
Lisha SUN
- Publication Type:Journal Article
- From:
China Pharmacy
2001;12(2):78-81
- CountryChina
- Language:Chinese
-
Abstract:
AIM：To investigated the effects of Ganoderma polysaccthride(GLB7)on protein kinase A(PKA) and protein kinase C(PKC) activitives in murine T cells.METHODS：A new ion-pair reversed-phase high liquid chromatography method was used to determinate the activities of PKA and PKC in T cells.RESULTS：GLB7 could markedly increase the activities of PKA and PKC in murine T cells in a dose-dependent manner.The peak time was at 5 min and 20 min and the activities of PKA and PKC returned to basic level at 20 min and 1.5h respectively.GLB7 could induce translocation of PKC and antagonize the inhibition effect of staurosporine(10μ mol· L-1) on PKC in T cells.CONCLUSION：The immunopotentiating and antitumour effects of Ganoderma polysacchride may be associated with its activation on PKA and PKC in murine T cells.
