Regulation of Hexokinase Activity by HB-EGF in Mesangial Cells
- VernacularTitle:HB-EGF对系膜细胞己糖激酶活性的调节
- Author:
Jianfei MA
;
Xijing ZHOU
- Publication Type:Journal Article
- From:
Journal of China Medical University
2001;30(2):91-93
- CountryChina
- Language:Chinese
-
Abstract:
Objective: Our aim was to study the concentrations and regulatory characteristics of heparin-binding epidermal growth factor-like factor (HB-EGF) and its functional consequences in inducing the activities of hexokinases (HKs) and net Glc utilization. Methods: The SV40MES13 cell line was used as cell model for this experiment to measure the total activity of Hks by using a standard G6PDH-coupled assay. The net Glc utilization was measured indirectly as Glc disappearance from the medium using the standard Glc oxidase-coupled reaction(Trinder). Results: The HB-EGF induced HKs activity in time- and dose-dependent manners and increased the net Glc utilization by SV40MES13 cells. Conclusion: The HB-EGF plays an important role in pathogenesis of diabetic nephropathy by inducing total HKs activity specifically and increases the net Glc utilization in mesangial cells.
