Study on Endonuclease Map of P1 Protein Gene of Mycoplasma Pneumoniae Isolated Strain in Shenyang
- VernacularTitle:沈阳地区肺炎支原体分离株P1蛋白基因限制性酶切图谱分型研究
- Author:
Guizhen WANG
;
Zhanshuang DONG
;
Zhongshun LIU
;
Baoqiang LI
- Publication Type:Journal Article
- From:
Journal of China Medical University
2001;30(2):103-104
- CountryChina
- Language:Chinese
-
Abstract:
Objective: Our aims were to classify mycoplasma pneumoniae (MP) clinical isolated strain according to endonuclease map of the PCR-products of the MP P1 protein gene and to investigate MP epidemic state in Shenyang.Methods:The P1 protein gene fragment of MP isolated strain was amplified by using PCR method ,and its products were digested with Hea III, 1.5 % argarose gel electrophoresis.Results:Isolated strain showed the same map as MP FH standard strain.Conclusion: Clinical isolated strain of mycoplasma pneumoniae in Shenyang belongs to groupⅡ.
