Effects of preconditioning and praeruptorine C on intracellular free calcium level and NO content in hypertrophied vascular smooth muscle cells with hypoxia
- VernacularTitle:预适应和前胡丙素对缺氧肥厚血管平滑肌胞内钙离子浓度及NO含量的影响
- Author:
Manren RAO
;
Wanbing LIU
;
Xiaowen ZHANG
- Publication Type:Journal Article
- From:Chinese Journal of Pharmacology and Toxicology
2001;15(2):141-144
- CountryChina
- Language:Chinese
-
Abstract:
Effects of preconditioning(PC) and praeruptorine C(Pra-C) on intracellular free calcium level(［Ca2+］i) and NO content in hypertrophied vascular smooth muscle cells (VSMC) with hypoxia were studied. ①The aorta VSMC of rats with renovascular hypertension induced by two-kidney-one-clip(2K1C) were isolated and cultured. Fura-2/AM was as a calcium fluorescent indicator. It was seen that Pra-C(20 mg*kg-1*d-1，ig, for 9 weeks from week 9 after 2K1C) and PC (3 cycles of 5 min pure N2 and 5 min 95%O2+5%CO2) antagonisted the elevated activities(higher ［Ca2+］i) induced by KCl and norepinephrine in VSMC with hypo- xia (30 min pure N2). ② In hypertrophied VSMC stimulated by angiotensinⅡ, combination of Pra-C(10 μmol*L-1 for 24 h) and PC enhanced NO content to the normal level in hypertrophied VSMC with hypo- xia. The results suggest that combination of Pra-C and PC have cooperative protection for hypertrophied VSMC with hypoxia injury.
