Placental Superoxide Dismutase Activity and Genetic Polymorphism in Preeclampsia and Normal Pregnancy.
- Author:
Mi Hye PARK
1
;
Young Joo KIM
;
Jung Ja ANN
;
Jong Il KIM
;
Sun Hee CHUN
Author Information
1. Department of Obstetrics and Gynecology, College of Medicine, Ewha Womans University, Seoul, Korea. ewhapmh@ewha.ac.kr
- Publication Type:Original Article
- Keywords:
Preeclampsia;
Malondialdehyde;
Mn-SOD activity and polymorphism
- MeSH:
Chromatography, Liquid;
Female;
Genotype;
Humans;
Malondialdehyde;
Oxidative Stress;
Placenta;
Polymorphism, Genetic*;
Pre-Eclampsia*;
Pregnancy*;
Risk Factors;
Superoxide Dismutase*;
Superoxides*
- From:Korean Journal of Perinatology
2006;17(2):166-172
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: Our purpose was to investigate urinary malondialdehyde (MDA), manganese superoxide dismutase (Mn-SOD) activity and polymorphism in placental tissues of women with preeclampsia and to evaluate oxidative stress in the pathophysiology of preeclampsia. METHODS: Urins and placental tissues were obtained from 20 normal and 20 preeclamptic women at 3rd trimester. Urinary MDA was assayed by an high performanance liquid chromatography (HPLC). The placental Mn-SOD activity was assayed by westen blotting and The placental Mn-SOD genotyping was assayed by PCR-RFLP. Data were analyzed statistically using Student's t-test and Chi-square test. RESULTS: 1) Urinary concentration of MDA was not significantly different in preeclampsia (4.43+/-2.37 ug/g) as compared with normotensive pregnancy (4.39+/-1.17 ug/g). 2) Preeclamptic women had similar Mn-SOD activity in placenta (1.04+/-0.04U/mL protein) as compared with normotensive pregnancy (1.44+/-0.34 U/mL protein). 3) No significant difference in the polymorphismthe of Mn-SOD genotype in placenta was observed between preeclampsia and normotensive pregnancy (X2=0.06, p>0.05) CONCLUSION: The findings in this study do not show that oxidative stress might be a pathogenetically relevent process causally contributing to the disease, and polymorphism in the Mn-SOD genotype in placenta do not seem to be risk factors for preeclampsia.
