Effect of γ-interferon on the expresson of Fas, Bcl-2 on Hep-G2 hepatocellular carcinoma cells
- VernacularTitle:γ-干扰素对肝癌细胞株Hep-G2 Fas,Bcl-2表达的影响
- Author:
Yansong ZHENG
;
Yongkun LIN
;
Zheng SHI
;
Xinsheng LU
- Publication Type:Journal Article
- From:
Chinese Journal of General Surgery
2001;10(2):139-141
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influence of γ-interferon (γ-IFN) on liver cancer cell line (Hep-G2). Methods Observing the expression of Fas and Bcl-2 by γ-IFN-pretreated Hep-G2 cells via immunohistochemical stain; subsequently treating these cells with adrimysin, and observing the cell death rate and apoptosis of these cell by MTT and electroscopy. Results (1) γ-IFN up-regulating the expression of Fas protein and down-regulating Bcl-2 protein (P<0.05), and the sensitivity of pretreated hep-G2 cell to adrimysin was increased. Conclusions γ-IFN can rise the sensitivity of Hep-G2 to adrimysin via regulating the expression of Fas and Bcl-2.
