Expression of kringlie 1～3 of angiostatin in Pichia and activity detection
- VernacularTitle:血管生成抑制素K1～3环结构体酵母表达及活性检测
- Author:
Ming SUN
;
Hongling ZHAO
;
Lichun WANG
;
Jiong WANG
;
Shaoqing HE
;
Qihan LI
- Publication Type:Journal Article
- From:
Chinese Journal of Biochemical Pharmaceutics
2001;22(2):55-57
- CountryChina
- Language:Chinese
-
Abstract:
Purpose The aim is to invest the mechanism and activity of kringle1～3 gene of angiostatin. Methods Kringle1～3 gene of angiostatin was abtained from hepatocyte of normal human by RT-PCR and inserted into expression vector pPIC9K in Pichia expression system. The recombinant protein was induced secrete , purified and test the activity by MTT.Results Krigle1～3 protein could inhibit vascular endothelial cell proliferative activity.Conclusion The reaction similar apoptosis induced by kringle1～3 recombinant protein of angiostatin inhibit cell proliferation.
