Purification of human urine erythropoietin and thrombomodulin
- VernacularTitle:人尿红细胞生成素和血栓调节素的纯化研究
- Author:
Yonghui TAO
;
Jian JIN
;
Rongjun ZHANG
;
Yang HE
;
Manda ZHANG
;
Changgeng RUAN
- Publication Type:Journal Article
- From:
Chinese Journal of Biochemical Pharmaceutics
2001;22(2):57-60
- CountryChina
- Language:Chinese
-
Abstract:
Purpose The aim is to isolate and purify the erythropoietin(EPO)and thrombomodulin(TM) from human urine.Methods Purifying and isolating human urine with means of filtration and concentration,ion-exchange chromatography, affinity chromatography and so on.Results 4.47 mg EPO and 9.92 mg TM were obtained from 2 000 kg human urine.Their molecular weights were(38.6±1.0)kD and (60±1.4)kD erespectively, the isoelectric point, 3.60±0.02 and 3.70±0.02, yield, 31.9% and 25.0%.The purified EPO and TM was shown to be highly homogeneous by capillary zone-electrophoresis(CZE),abundant Glu，Ala and Gly include on TM. Conclusion The high purity EPO and TM was obtained.
