The effects of rhIL-11 on hematopoietic restoration following cyclophosphamide-induced thrombocytopenia in cynomolgus monkeys
- VernacularTitle:重组人白细胞介素-11对环磷酰胺致猕猴血小板减少模型的作用
- Author:
Shuqin YU
;
Zhenli ZHANG
;
Yong SUN
;
Jian TONG
- Publication Type:Journal Article
- From:
Chinese Journal of Biochemical Pharmaceutics
2001;22(2):61-64
- CountryChina
- Language:Chinese
-
Abstract:
Purpose The aim is to evaluate the effects of rhIL-11 made in China on hematopoietic recovery following chemotherapy-induced thrombocytopenia in cynomolgus monkeys.Methods Chemotherapy-induced myelosuppression of cynomolgus monkeys was made by china iv cyclophosphamide ( 30 mg/kg, qd ) for 5 days, then animals were divided 6 groups(n=4), by sc rhIL-11( 50, 100, 200 μg/kg), or Neumega (GI rhIL-11 control, 100 μg/kg) for 14 days, another normal control and model control. Blood was taken before chemothery and then at day 3, 6,9,12,15,18,21 for blood cell counts and platelet congregate test. Bone marrow was aspirated day 0, 12 and 21 for evaluation of the megakaryocyte ploidy distribution.Results Recovery of blood platelets was accelerated and reached normal levels by day 12, and was higher than normal day 18, day 21 sc rhIL-11 in cynomolgus moneys. Blood platelet congregated rate were 71.4%～74.6% by day 21 and higher than model control . megakaryocytes in bone marrow increased.Conclusion rhIL-11 could accelerate the recovery of peripheral blood platelets in monkeys. The function of increased platelets was normal. The results supported the clinical use of rhIL-11 as a platelet restorative agent to prevent severe thrombocytopenia following chemotherapy.
