A method of on-column sample stacking in capillary electrophoretic analysis of micro-scale amino acids
- VernacularTitle:毛细管电泳测定微量氨基酸柱上样品堆积法研究
- Author:
Zuojun SHEN
;
Zengpei SUN
;
Shude YANG
- Publication Type:Journal Article
- From:
Chinese Journal of Biochemical Pharmaceutics
2001;22(2):71-73
- CountryChina
- Language:Chinese
-
Abstract:
Purpose The aim is to develop a method for the analysis of micro-scale amino acids in biological samples.Methods After having been extracted and dried,the residues were dissolved in a running buffer or a distilled water and acetonitrile.The samples were loaded into column for 5,25 or 50s at a hydrodynamic injection and were separated by capillary electrophoresis.The effect of different dissolvent on the sample stacking was observed.Results A 100-fold improvement in the amount of material that can be injected into a capillary column without loss of resolution was shown.Conclusion The marked effect of sample stacking was achieved by the using distilled water and acetonitrile(1∶1,v/v) as dissolvent.
