Determination of floxuridine in plasma by HPLC
- VernacularTitle:高效液相色谱法测定人血浆中氟脲脱氧核苷的浓度
- Author:
Pingfang HUANG
;
Changlian WANG
;
Yiwei LIU
;
Xi CHEN
- Publication Type:Journal Article
- From:
Chinese Journal of Biochemical Pharmaceutics
2001;22(2):84-85
- CountryChina
- Language:Chinese
-
Abstract:
Purpose The aim is to establish the HPLC method for the determination of Fluoro-deoxyuridine in plasma.Methods The Chromatography conditions include: Chromatography column: Nova-pak C18(3.9mm×150mm,4μm), mobile phase: 0.05mol/L sodium phosphate monobasic -methanol-water(0.5∶7∶92.5)， UV detection at 260nm， FUDR was extracted with ethyl acetate. Results The average recoveries were 96.4%，96.5%，97.8% for concentration 0.23、1.67、20.0μg/ml (n=5).The corresponding reproducibility were RSD 1.61%, 1.98%, 3.17% respectwely for iner-day and RSD 3.56%, 1.90%, 2.63% for the intra-day(n=5). The FUDR concentration was linear with a correlation coefficient of 0.999 4 over the range of 0.099～20.0μg/ml. Conclusion The method was sensitive and accurate and suitable for pharmacokinetics and bioavailability study of FUDR.
