Detection of Wilson disease gene expression in transfected Menkes disease patients fibroblast.
- VernacularTitle:肝豆状核变性基因在皮肤成纤维细胞株转染后的表达
- Author:
Zhenwen YAN
;
Xiuling LIANG
;
Chunshui YANG
;
Guoqing HOU
;
Pingyi XU
;
Rong CHEN
;
Zhiheng HUANG
;
Fan HUANG
- Publication Type:Journal Article
- From:
Chinese Journal of Nervous and Mental Diseases
2001;27(2):83-85
- CountryChina
- Language:Chinese
-
Abstract:
Objective Determination of Wilson disease gene mRNA expression in human fibroblast cell strain (Me32aT22/2L) by reverse transcription-polymerase chain reaction (RT-PCR). Methods Using lipofection reagent, the plasmid vector carrying the Wilson disease gene (pRc/CMV-WD) was transferred into Me32aT22/2L cultured in serum free complement medium. RT-PCR was used to determine WD mRNA expression in Me32aT22/2L. Results Wilson disease gene expression was detected in Me32aT22/2L, while no specific signals were detected in untransfected fibroblast. Conclusions It demonstrated that Me32aT22/2L strain could express the Wilson disease gene, suggesting that Wilson disease gene transfer might develop a new approach to study Wilson disease.
