A preliminary study of the molecular mechanism of Cryptococcus neoformans mutation in the production of melanin

VernacularTitle:新生隐球菌白化突变分子机制的初步研究
Author: Hong SANG ; Wanqing LIAO ; Jianghan CHEN ; Hai WEN
Publication Type:Journal Article
From: Journal of Medical Postgraduates 2001;14(2):103-105
CountryChina
Language:Chinese
Abstract: Objectives：To study the molecular mechanism of Cryptococcus neoformans mutation in the production of melanin.Methods：By comparing wild types(Mel+) and mutants(Mel-),we detected C.neoformans mutant laccase (CNLAC1) gene by PCR.We also cloned gene of the CNLAC1 containing about 450bp in length.E.coli DH5α was the host strain for the plasmid containing the DNA clone.Results：No deletion was found in wild type.However,CNLAC1 deletion (about 450 bp) was present (between 1 715 bp and 2 617 bp).CNLAC1 containing about 450 bp in length was cloned successfully.Conclusions：The present study was helpful in the biochemical and molecular study of CNLAC1 mutation.It might also provide us a target gene for the prognosis and gene therapy of Cryptocosis.