Preparation of components of defensins from human and rabbit neutrophils by continuous acid-urea-polyacrylamide gel electrophoresis (CAU-PAGE)
- VernacularTitle:连续酸-尿素-聚丙烯酰胺凝胶电泳制备人和兔中性粒细胞防御素各组分
- Author:
Yongjie WANG
;
Ming QI
- Publication Type:Journal Article
- From:
Journal of Medical Postgraduates
2001;14(2):106-109
- CountryChina
- Language:Chinese
-
Abstract:
Objectives:To develop a preparative method of continuous acid-urea-polyacrylamide gel electrophoresis (CAU-PAGE) to purify the defensin components from human and rabbit neutrophils (HNP-1～3 and RNP-1～5).Methods:Neutrophils were isolated from human peripheral blood or rabbit artificial aseptic ascites.The components of defensin were extracted by 5% acetic acid directly followed by elution of CAU-PAGE,and purified HNP-1～3 or RNP-1～5 were harvested.Polyacrylamide gel filtration was used as the method for comparison.Results:For each elution circa 1.50 mg of purified HNP or 2.50 mg of RNP were obtained from 5×108 of neutrophils,and their natural biological activity was preserved.Conclusions：CAU-PAGE is a relatively simple,rapid,economical method for purifying cationic peptides as the components of defensin.
