Experience of thin layer chromatography separation and bioassay of platelet activating factor
- VernacularTitle:血小板激活因子薄层层析分离和生物法测定的应用体会
- Author:
Weiming ZHU
;
Xi LI
;
Weifeng TU
;
Fangnan LIU
;
Yisheng YUAN
;
Jieshou LI
- Publication Type:Journal Article
- From:
Journal of Medical Postgraduates
2001;14(2):110-112
- CountryChina
- Language:Chinese
-
Abstract:
Objectives:To introduce the author's experiences in bioassay of platelet activating factor with thin layer chromatographic separation and platelet aggregation method,and to discuss probable problems in the process.Methods and Results:The method was used in determining portal venous blood samples from swine models of acute severe pancreatitis,control value were 0.79~1.65 pmol/L,in acute severe pancreatitis,mean blood level of platelet activating factor may be as high as 14.75 pmol/L.Conclusions:Thin layer chromatography separation and platelet aggregation assay were suitable for the determination of platelet activating factor,the results were satisfactory in case of standardized operation.