Polymerase chain reaction in determining telomerase activity in tumor tissues
- VernacularTitle:聚合酶链反应检测肿瘤组织的端粒酶活性
- Author:
Yiqin LUO
;
Wei YU
;
Hua TAN
;
Jianguo WU
- Publication Type:Journal Article
- From:
Journal of Medical Postgraduates
2001;14(2):112-114
- CountryChina
- Language:Chinese
-
Abstract:
Objectives:To detect telomerase activity in various tumor tissues by PCR-PAGE and PCR-MPH.Methods:We used telomerase PCR-MPH，an assay in which a digoxigenin-labeled telomerase repeat-specific probe was hybridized to the PCR product.After revelation with an anti-digoxin-AKP conjugates,the amount of hybridized probe was determined by optical reading.Telomerase activity was analyzed by PAGE.Results:Telomerase activity was determined by PCR-MPH accurately and specifically in various tumor tissues,its sensitivity was one hundred times higher than that of PCR-PAGE.Conclusion:PCR-MPH is ideal for analyzing telomerase activity in various tumor tissues.It is simple,easy and suitable for clinical application.
