Altered expression of SOCS-3 and GHR gene and its relation with growth hormone resistance in intraabdominal infection
- VernacularTitle:腹腔感染时SOCS-3和GHR mRNA表达的变化与生长激素拮抗
- Author:
Ping WANG
;
Ning LI
;
Jieshou LI
- Publication Type:Journal Article
- From:
Journal of Medical Postgraduates
2001;14(2):123-126
- CountryChina
- Language:Chinese
-
Abstract:
Objectives:To detect the altered expression of SOCS-3 and GHR gene in intraabdominal infection and its relationship to growth hormone resistance.Methods:Liver expression of SOCS-3,GHR and IGF-I mRNA were detected by RT-PCR in control and intraabdominal infected rats,serum growth hormone levels were measured by RIA in two groups.Results:Serum concentrations of growth hormone were elevated more obviously in infected rats than controls,they were (0.843±0.17)ng/ml and (0.614±0.138)ng/ml respectively,the difference was significant (P<0.05).Liver GHR mRNA levels were decreased by 57.2% in infected rats compared with control rats.SOCS-3 mRNA was weakly expressed in control rats,however,it was strongly expressed in infected rats and had a 312.8 % increase.Liver IGF-I mRNA levels were reduced by 45.3% in infected rats compared with control rats.Conclusions:The elevated SOCS-3 and reduced GHR mRNA expression may contribute to the growth hormone resistance in intraabdominal infection.
