BIODEGRADABLE POLY (D,L-LACTIDE-CO-GLYCOLIC ACID) MICROSPHERESUSED AS ADJUVANT IN DNA IMMUNIZATION OF HEPATITIS B VIRUS S GENE
- VernacularTitle:生物可降解微球作为乙型肝炎基因免疫佐剂的研究
- Author:
Fei GONG
;
Shude JIANG
;
Weidong LI
;
Junying CHEN
- Publication Type:Journal Article
- From:
Microbiology
2001;28(2):68-72
- CountryChina
- Language:Chinese
-
Abstract:
Study the immunological adjuvant function of biodegradable microspheres for DNA immunization. Empty poly(D,L-lactide-co-glycolic acid) microspheres were prepared using the water-inoil-in-water(w-o-w)technique; A plasmid DNA pRc-CMV encoding hepatitis B virus S antigen was constructed;The mixture of the microspheres and the plasmid DNA was prepared by incubation method. The mixture was administered to Balb/c mice by intramuscular injection. Result: The high antibody titer (1:1600) of intramuscular injection of the mixture of microspheres and the plasmid DNA was obtained, similar to that of intramuscular injection of the mixture of AL(OH)3 and hepatitis B virus S antigen; while intramuscular injection the plasmid DNA elicited no serum antibody respones. Conclusion: biodegradable microspheres may be used as an good adjuvant for DNA immunization.
