Study on Preparation and Identification of Immobilized Metal Ion Affinity Adsorption Gel
- VernacularTitle:固定化镍离子亲和层析胶的制备及其性能鉴定
- Author:
Ping LIU
;
Shuangquan ZHANG
;
Xiaomei YAN
;
Jinshu XU
- Publication Type:Journal Article
- Keywords:
Ni2+-IDA affinity gel;
atomic absorption;
activation;
chelation
- From:
Progress in Biochemistry and Biophysics
2001;28(2):267-269
- CountryChina
- Language:Chinese
-
Abstract:
Sepharose 6B was activated by epichlorohydrin in the strong base condition, and then reacted with solution of iminodiacetic sodium. The arms of IDA were conjuncted to the activated Sepharose 6B. Then the products were reacted with the solution of NiSO4. The arms of IDA were chelated with Ni2+,and the chelating resin―Ni2+-IDA could be prepared. The physicochemical indexes and performance in purifying protein of the expressing product were assayed with atomic absorption method and purifying aimed protein-human B lymphocyte stimulator(hBLyS) from the expressing products in E.coli. The results indicated that the performance of made gel is very good, and its price is less than 1/10 of that of commodity gel.
