Amino Acid Composition of the α Chain of Hemoglobin and Amino Acid Sequence of it′s Particular Peptide Fragment From 615 Mouse
- VernacularTitle:615小鼠血红蛋白α链的氨基酸组成及个别肽段的氨基酸序列
- Author:
Jinxia WU
;
Heying ZHANG
;
Jianping WANG
;
Jingcai WU
- Publication Type:Journal Article
- Keywords:
615 mouse;
hemoglobin;
amino acid composition;
amino acid sequence
- From:
Progress in Biochemistry and Biophysics
2001;28(2):259-262
- CountryChina
- Language:Chinese
-
Abstract:
The α chain of hemoglobin of 615 mouse was isolated and purified on CM-Celullose-23 colomn chromatography. The N-terminal amino acid of the α chain was valine determined with DABITC/PITC method.The amino acid composition was determined and it was different from the parent(C57BL)in literature on the number of leucine residue,histine residue and valine residue.An undissoluble ‘core’ and dissoluble peptides were found when the α chain of 615 mouse was hydrolysised by trypsin and it was found that the eighth amino acid residue from N-terminal of one particular peptide fragment mutated from valine (C57BL) to leucine.
