The Gene Cloning, Expression and Bioactivity of the Human FKBP52
- VernacularTitle:人FKBP52的基因克隆、表达及活性研究
- Author:
Wuhong PEI
;
Yonghuai HE
;
Xing CHEN
;
Song LI
;
Beifen SHEN
- Publication Type:Journal Article
- Keywords:
hFKBP52;
gene clone;
proyoatic expression;
peptidyl-pr olyl cis-trans isomerase
- From:
Progress in Biochemistry and Biophysics
2001;28(2):236-239
- CountryChina
- Language:Chinese
-
Abstract:
To obtain active hFKBP52 protein for screening novel neu rotrophic drugs. Semi-nested and overlap PCR and affinity chromatography were u sed. hFKBP52 gene was cloned successfully from human fetal brain cDNA library, a nd then highly expressed (about 30%) as fusion protein in pET28a(+) vector syste m. The recombinant protein was purified as one band on SDS-PAGE. The purified h FKBP52 showed peptidyl-prolyl cis-trans isomerase (PPIase) activity, simil ar to the wild type.
