Primary Studies on the Progression of S Phase and its Molecular Mechanism by Protein Kinase A Inhibitor in the HeLa Cell
- VernacularTitle:蛋白激酶A抑制剂对HeLa细胞S期进程的影响
- Author:
Xia SUN
;
Huitu LIU
;
Yingkai TONG
;
Duanshun WANG
- Publication Type:Journal Article
- Keywords:
protein kinase A;
protein kinase A inhibitor;
S phase;
HeLa cells
- From:
Progress in Biochemistry and Biophysics
2001;28(2):232-235
- CountryChina
- Language:Chinese
-
Abstract:
The synchronized HeLa cells were used to study the effect of protein kinase A(PKA) inhibitor on the progression of S phase. Synchronized cell s in S phase were obtained by the method of TdR double block through 3H-T dR incorporation assay. The PKA inhibitor typeⅢ obviously increased the level of 3H-TdR incorporation of S phase in HeLa cells. In contrast with contro l, the activity of thymidine kinase (TK) in S phase increased, too. It indicated that PKA played an inhibitory role in S phase progression of HeLa cells. With t he method of Western blotting, the PKA inhibitor typeⅢ enhanced the level of C yclinA and PCNA, inhibited the expression of p21, which is a negative regulator of cell cycle, but had no effect on the expression of CDK2. The results showed t hat PKA could negatively regulate the S phase progression by affecting the level of CyclinA, PCNA and influencing the expression of p21 protein. This may be one of the molecular mechanisms which is involved in the negative regulation of S p hase progression by PKA in HeLa cells.
