Effect of PMA on Growth and Apoptosis of Human Melanoma Cell Overexpressing CKI p15INK4B
- VernacularTitle:佛波酯对CKI p15INK4B高表达的人黑色素瘤细胞生长及凋亡的影响
- Author:
Yingkai TONG
;
Huitu LIU
;
Jun LIU
- Publication Type:Journal Article
- Keywords:
p15;
CKI;
PMA;
PKC;
apoptosis
- From:
Progress in Biochemistry and Biophysics
2001;28(2):198-202
- CountryChina
- Language:Chinese
-
Abstract:
The plasmid pXJ-41-p15, which contains the full length DNA coding for p15 was introduced into human melanoma cell line A375 in which p 15 was deleted by DNA recombination and transfection. Using G418, the positive clones were selected. And the cell model overexpressing p15 was constructed suc cessfully through the analysis of PCR and Western blot. It is showed tha t the expression of p15 was further enhanced after the cells overexpressing p15 were treated with PMA for 72 hours. In contrast of the control cells, the PKC a ctivity was further declined in the cells overexpressing p15 after treated with PMA. At the same time, the growth rate of cell was decreased more significantly and approximate 30% apoptotic cells were found. The expression of Caspase-3(P2 0) was increased in the apoptotic cells. It is indicated that CKI p15 was relat ed to PKC signal transduction in the regulation of cell proliferation and apopto sis. They may be involved in the apoptotic pathway including Caspase3, thus indu cing the apoptosis of cells.
